Name Nik Azma is proud to introduce new partnership with Diatech Pharmacogenetics, manufacturer of Kit linea Easy® for cancer mutation detection. We are exclusive partner of Diatech for Iran market.
Kit linea Easy®
The Easy® kits allow the qualitative detection of the main somatic mutations of the genes EGFR, KRAS, NRAS and BRAF by Real-Time PCR in association with a system of enrichment of the mutated allele.
The kit Easy® ALK is designed for the qualitative detection of the aberrant expression of the tyrosin-kinase domain of ALK, which is caused by events of gene rearrangement, by One Step Real-Time RT-PCR.
Each kit includes all the reagents necessary for the test and the positive controls of reaction.
The kits are validated on the following instruments:
▪ Rotor-Gene Q ▪ Rotor-Gene 6000
▪ Stratagene Mx3000P ▪ Stratagene Mx3005P
▪ ABI 7300 ▪ Bio-Rad CFX96
All the kits (on each of the above listed instruments) share the same thermal profile for the detection of the somatic mutations.
1- Easy® KRAS (24 test, CE IVD Real Time PCR)
Main features:…………………………………………………………
Detection of the main mutations of exon 2 (codons 12, 13), of exon 3 (codons 59, 61) and of exon 4
(codons 117, 146) of the gene KRAS using 12 oligo mixes.
Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene.
A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA
in each sample.
Sensitivity:………………………………………………………………
The kit allows the detection of low percentages of mutated allele in presence of high amounts of wildtype
genomic DNA by real-time amplification with sequence-specific probes marked with FAM and
HEX (LOD down to 0.5%)
Sample:……………………………………………………………………
The kit allows the analysis of DNA extracted from fresh, frozen and formalin-fixed paraffin-embedded
tissues
Execution time:………………………………………………………..
2 hours
2- Easy® BRAF (24 test, CE IVD Real Time PCR)
Main features:…………………………………………………………
Detection of the main mutations of codon 600 of the gene BRAF using 5 oligo mixes.
Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene.
A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each
sample.
Control DNA positive for all the mutations detected by the kit.
Sensitivity:………………………………………………………………
The kit allows the detection of low percentages of mutated allele in presence of high amounts of
wild-type genomic DNA by rea cific probes marked
with FAM and HEX (LOD down to 0.5%).
Sample:……………………………………………………………………
The kit allows the analysis of DNA extracted from fresh, frozen and formalin-fixed paraffin-embedded
tissues
Execution time:………………………………………………………..
2 hours
3- Easy® EGFR (24 test, CE IVD Real Time PCR)
Main features:…………………………………………………………
Detection of the main mutations of exons 18, 19, 20, 21 of EGFR gene using 8 oligo mixes.
Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control
gene.
A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA
in each sample.
Sensitivity:………………………………………………………………
The kit allows the detection of low percentages of mutated allele in presence of high amounts of
wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAM
and HEX (LOD down to 0.5%).
Sample:……………………………………………………………………
The kit allows the analysis of DNA extracted from fresh, frozen and formalin-fixed paraffin-embedded
tissues and of circulating tumor DNA extracted from plasma
Execution time:………………………………………………………..
2 hours
4-Easy® NRAS (24 test, CE IVD Real Time PCR)
Main features:…………………………………………………………
Detection of the main mutations of exon 2 ( codons 12, 13), of exon 3 (codons 59, 61) and of exon 4
(codons 117, 146) of NRAS gene of EGFR gene using 8 oligo mixes.
Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control
gene.
A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each
sample
Sensitivity:………………………………………………………………
The kit allows the detection of low percentages of mutated allele in presence of high amounts of
wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAM
and HEX (LOD down to 0.5%)
Sample:……………………………………………………………………
The kit allows the analysis of DNA extracted from fresh, frozen and formalin-fixed paraffin-embedded
tissues
Execution time:………………………………………………………..
2 hours
5-Easy® ALK (24 test, CE IVD Real Time PCR)
Main features:…………………………………………………………
Qualitative detection of aberrant expression of the tyrosin-kinase domain of ALK associated to
the gene fusion by retrotrascription and co-amplification of a region located at 3’ terminus of ALK
mRNA (detected by a FAM marked probe) and of an endogenous control gene (detected by
a HEX marked probe).
Sample:……………………………………………………………………
The kit allows the analysis of RNA extracted from fresh, frozen or formalin-fixed paraffin embedded
tissues.
Execution time:………………………………………………………..
2 hours
6-Easy® DPYD (24 test, CE IVD Real Time PCR)
Main features:…………………………………………………………
Detection, by allelic discrimination, of the DPYD gene polymorphisms DPYD*2A (IVS14+1G>A, c.1905+1G>A, rs3918290), DPYD*13 (c.1679T>G, rs55886062), DPYD D949V (c.2846A>T, rs67376798) and DPYD IVS10 (c.1129–5923C>G, rs75017182), associated with the toxicity due to the treatment with fluoropyrimidines, using 4 oligo mixes.
Each mix allows the co-amplification of the mutant sequence (FAM) as well as the wild-type sequence (HEX).
Sample:……………………………………………………………………
The kit allows the analysis of genomic DNA extracted from whole blood
Execution time:………………………………………………………..
2 hours